Identification and molecular characterization of brucella strains isolated from sheep and goats in the West Bank, Palestine
العناوين الأخرى
تشحيص و دراسة جرثومة البروسيلا في الأغنام و المعز المتواجد في منطقة الضفة الغربية، فلسطين تقانات الأحياء الجزيئية.
مقدم أطروحة جامعية
مشرف أطروحة جامعية
Isawi, Tamir
Farraj, Muhammad A.
أعضاء اللجنة
Adwan, Kamil M.
Rappocciolo, Emilia
الجامعة
جامعة بيرزيت
الكلية
كلية العلوم
القسم الأكاديمي
دائرة الأحياء و الكيمياء الحيوية
دولة الجامعة
فلسطين (الضفة الغربية)
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2010
الملخص الإنجليزي
Serum samples were obtained from 350 animals comprising of 302 sheep and 48 goats with signs of brucellosis from different localities in West Bank, Palestine None of the animals were vaccinated against Brucella.
These samples were subjected to serological examination for the detection of specific brucella antibodies.
The percentage of the positive brucellosis sera was found to be as follows : 31 % in sheep sera and 52 % in goats sera by Rose Bengal test (RBT).
giving overall seroprevalence of 34 %.
The overall Seropositivity using Complement Fixation Test (CFT) was 31 % ; 29 % in sheep sera and 44 % in goats sera.
Eighty milk samples collected from seropositive animals were subjected to bacteriological examination.
Brucella organisms were detected in 38 (47.5 %) of the samples.
Testing the milk samples by polymerase chain reaction (PCR), all the 38 positive samples detected by bacterial examination were also detected by PCR.
Furthermore, with the PCR, we were able to additionally detect 24 (30 %) infected milk samples that were negative by the bacterial isolation method.
B.
melitensis was identified from 42 out of 80 milk samples.
The hem agglutinin gene sequence of two of the B.
melitensis genes was PCR-amplified using the primers ORF and IS711, sequenced and subsequently aligned with other Gen Bank-accessible gene sequences of B.
melitensis and other Brucellae spp.
using version 2.0 of BLAST.
These sequences were identical to that of the recent sequence of type strain of B.
melitensis (ATCC 23456).
Nucleotide comparison and restriction enzyme analysis of hem agglutinin revealed that the two current isolates were different from vaccine (Rev.1).
The results of phylogenetic analysis revealed that they were genetically close to the isolates from different Mediterranean countries, particularly those from France, Spain and Israel.
التخصصات الرئيسية
الموضوعات
عدد الصفحات
92
قائمة المحتويات
Table of contents.
Abstract.
Chapter One : introduction.
Chapter Two : materials and methods.
Chapter Three : result.
Chapter Four : disscussion.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Awwad, Ilina Alixi. (2010). Identification and molecular characterization of brucella strains isolated from sheep and goats in the West Bank, Palestine. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-303595
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Awwad, Ilina Alixi. Identification and molecular characterization of brucella strains isolated from sheep and goats in the West Bank, Palestine. (Master's theses Theses and Dissertations Master). Birzeit University. (2010).
https://search.emarefa.net/detail/BIM-303595
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Awwad, Ilina Alixi. (2010). Identification and molecular characterization of brucella strains isolated from sheep and goats in the West Bank, Palestine. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-303595
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-303595
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر