Identification and molecular characterization of brucella strains isolated from sheep and goats in the West Bank, Palestine

العناوين الأخرى

تشحيص و دراسة جرثومة البروسيلا في الأغنام و المعز المتواجد في منطقة الضفة الغربية، فلسطين تقانات الأحياء الجزيئية.

مقدم أطروحة جامعية

Awwad, Ilina Alixi

مشرف أطروحة جامعية

Isawi, Tamir
Farraj, Muhammad A.

أعضاء اللجنة

Adwan, Kamil M.
Rappocciolo, Emilia

الجامعة

جامعة بيرزيت

الكلية

كلية العلوم

القسم الأكاديمي

دائرة الأحياء و الكيمياء الحيوية

دولة الجامعة

فلسطين (الضفة الغربية)

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2010

الملخص الإنجليزي

Serum samples were obtained from 350 animals comprising of 302 sheep and 48 goats with signs of brucellosis from different localities in West Bank, Palestine None of the animals were vaccinated against Brucella.

These samples were subjected to serological examination for the detection of specific brucella antibodies.

The percentage of the positive brucellosis sera was found to be as follows : 31 % in sheep sera and 52 % in goats sera by Rose Bengal test (RBT).

giving overall seroprevalence of 34 %.

The overall Seropositivity using Complement Fixation Test (CFT) was 31 % ; 29 % in sheep sera and 44 % in goats sera.

Eighty milk samples collected from seropositive animals were subjected to bacteriological examination.

Brucella organisms were detected in 38 (47.5 %) of the samples.

Testing the milk samples by polymerase chain reaction (PCR), all the 38 positive samples detected by bacterial examination were also detected by PCR.

Furthermore, with the PCR, we were able to additionally detect 24 (30 %) infected milk samples that were negative by the bacterial isolation method.

B.

melitensis was identified from 42 out of 80 milk samples.

The hem agglutinin gene sequence of two of the B.

melitensis genes was PCR-amplified using the primers ORF and IS711, sequenced and subsequently aligned with other Gen Bank-accessible gene sequences of B.

melitensis and other Brucellae spp.

using version 2.0 of BLAST.

These sequences were identical to that of the recent sequence of type strain of B.

melitensis (ATCC 23456).

Nucleotide comparison and restriction enzyme analysis of hem agglutinin revealed that the two current isolates were different from vaccine (Rev.1).

The results of phylogenetic analysis revealed that they were genetically close to the isolates from different Mediterranean countries, particularly those from France, Spain and Israel.

التخصصات الرئيسية

علم الحيوان
الطب البشري

الموضوعات

• مرض البروسيلات
• الضفة الغربية
• الأغنام
• الماعز
• التشخيص

عدد الصفحات

92

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-303595