Genetic diversity of different Jordanian fig(ficus carica l.)‎ Germplasm revealed by morphological traits and ISSR markers

العناوين الأخرى

التباين الوراثي في الأصول الوراثية للتبن من الأردن باستخدام الصفات الشكلية و البصمة الوراثية (ISSR)‎

مقدم أطروحة جامعية

al-Majali, Duaa Abd al-Hamid

مشرف أطروحة جامعية

Abd al-Ghani, Adil Hasan Mahmud

أعضاء اللجنة

Miqdadi, Husayn Muhammad
al-Absi, Khalid Musa
al-Hunayti, Dukhi Abd al-Rahim

الجامعة

جامعة مؤتة

الكلية

كلية الزراعة

القسم الأكاديمي

قسم الإنتاج النباتي

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2009

الملخص الإنجليزي

Variation of twenty four accessions belonging to the 7 fig landrace and 6 fig wild genotypes were investigated at the phenotypic level with 32 morphological traits and at the DNA level using Inter Simple Sequence Repeats (ISSR) marker.

Broad genetic diversity was existed among Jordanian fig germplasm.

Fig landraces and fig wild type group possessed desirable traits, which serve as target for growers and breeders such as fruit weight, pulp flavor, ease of peeling and resistance to ostioleend cracks.

A total of 22 DNA markers were scored using 5 ISSR primers over 24 landrace accessions and 6 fig wild type genotypes, with a mean of 4.0 markers per primer and 90% mean level of the proportion of polymorphic loci indicating a large genetic diversity within the local Jordanian fig germplasm.

The correlation between the pair-wise distances estimated from ISSR and the Euclidean genetic distances from morphological traits were found to be non-significant, which represents nearly no association and indicates there is no consistency between these two marker systems.

However, some common groupings were observed in ISSR and morphological traits based clustering.

'Kdaree', 'Hmaree' and some Byadee' landraces that were closely clustered in ISSR based dendogram, were also clustered in the morphology based dendogram.

Clones derived from 'Kortomanee' and 'Zrakee' were also closely clustered in both analyses.

Wild fig had also somewhat close clustering in ISSR and morphological traits based clustering.

'Ajlounee' and 'Byadee' landraces accessions were distributed in different clusters possibly due to their genetic variability and / or miss-naming.

Clustering based on ISSR data produced one large cluster and one small cluster containing one clone from 'Ajlounee' cultivar 'Aj3' and one wild fig genotype 'W1'.

Similarly, clustering based on morphological data placed fig wild type genotype 'W3' in one isolated main cluster.

These results may indicate the unique genetic background of these accessions.

Principle component analysis revealed that first five principle components accounted for 28.81, 16.55, 12.13, 9.03and 7.66 %, respectively for all morphological data ; whereas principle component analysis ISSR markers, explained 20.43, 20.01 and 12.93 % of the total variation, respectively.

Results obtained from this study suggested that the most effective traits that explained the variability among Jordanian fig germplasm were fruit weight and width, fruit flesh thickness, fruit skin ground color, résistance to ostiole end-cracks, color of scales around ostilum, fruit lenticels quantity, uniformity of fruit size, fruit apex shape, shape of fruit stalk, easiness of peeling, fruit length and leaf length, width and area, shape of leaf base, petiole color, location of little lateral lobes and leaf color, respectively.

Clustering obtained by principle component analysis and principle coordinate analysis using morphological data and ISSR markers, respectively, were found to be consistent with those obtained through UPGMA clustering analyses.

The existence of intra-cultivar polymorphism was shown by marker differences and differences in quantitative traits between fig accessions from the same landrace.

The same marker combination occurred among accessions collected from 'Mwazee' and 'Zrakee' landraces.

However, different marker combinations observed among clones collected from Kortomanee', 'Ajlounee', 'Kdaree', 'Byadee' and 'Hmaree' landrace s as well as among fig wild type.

التخصصات الرئيسية

النبات

الموضوعات

• الأردن
• الجوانب الوراثية

عدد الصفحات

124

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-304548