The phytotherapeutic effect of traditional crude oil of nigella sativa on male reproductive system of albino mice treated with low toxic dose of paracetamol

الملخص EN

The study included (20) adult male Swiss albino mice Balb / C, they were randomly divided into four groups : the first was injected intra peritoneal with 300 mg / kg body weight (B.W.) of paracetamol which considered as low toxic dose, followed by 0.3 ml of normal saline(0.9 % w / v) was administrated orally .

Second, mice administrated orally with 0.3 ml of crude traditional oil of Nigella sativa, then injected intra peritoneal with 0.3 ml of normal saline (0.9 % w / v).

Third, mice injected intra peritoneal with 300 mg / kg (B.W.) of paracetamol, then administrated orally with 0.3 ml of crude traditional oil of Nigella sativa.

Fourth the control group, mice injected intra peritoneal with 0.3 ml of normal saline (0.9 % w / v), then administrated orally again with 0.3 ml of normal saline.

The experimental time of treatment was 24 hours for all groups, then all animals were sacrificed, the reproductive organs were dissected out for weighing then preserved in Bouins fixative for histopathy.

Statistical analysis showed a significant increase (P < 0.05) in the value of GPT ( 0.043 ± 0.017) / IU , in the mice treated with 300 mg / kg (B.W) of low toxic dose of paracetamol in compare with control and other treated groups .

Also, there is a significant increase (P < 0.05) in the weight of seminal vesicle (0.213 ± 0.058) / gm in mice administrated with 0.3 ml of crude oil of Nigella sativa as compare with control and other treated groups.

Mice group administered with 0.3 ml of crude oil Nigella sativa revealed highly significant increase in sperm count (336 ± 100.17 * 104 ) sperm / mm3 at (P < 0.01 ) as compared with control and other treated groups Hypsometry of reproductive organs, revealed a significant increase (P < 0.05) in the wall thickness of testicular seminiferous tubules (66 ± 26.8) / μ in mice administrated with 0.3 ml of crude oil Nigella sativa , in contrast to control group and other treatments.

Whereas, the diameter of seminiferous tubules and the height of glandular epithelia of seminal vesicle never be affected either by the administration of crude oil of Nigella sativa nor with the injection of 300 mg / kg (B.W) of low toxic dose of paracetamol.

The weights of right and left testis and prostate gland never are affected with all treatments that included in the current study.