Cadmium-resistant bacteria isolated from Petra city wastewater treatment station

العناوين الأخرى

عزل بكتيريا مقاومة للكادميوم من محطة تنقية المياه العادمة لمدينة البتراء جنوب الأردن

مقدم أطروحة جامعية

Ajrud, Nawal Rashid

مشرف أطروحة جامعية

Khulayfat, Khalid Muhammad
Nawayisah, Khalid

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2009

الملخص الإنجليزي

A Klebsiella oxytoca strain was isolated from Petra City Wastewater Treatment Plant.

A single plasmid (>23 kb) was detected in this strain and transformed into E.

coli JM83.

The transformed E.

coli cells exhibited elevated resistance to cadmium as compared to parental plasmid-free cells.

The SD Striated cells showed higher efficiency in plasmid curing than ethidium bromide treated cells.

The ethidium bromide-cured cells grew only on 10 μg / mL Cd+2 as minimal tolerable concentration whereas the SDS-treated cells had no growth on any of Cd concentrations tested (5, 10, 20, 30, 40 and 50 μg / mL).

Contrary to Freundlich model, Langmuir model gave a good fit to the Cd bio sorption data by K.

oxytoca cells.

Plasmid curing caused 80, 82 and 70 % inhibition in the Cd bio sorption, adsorption and uptake, respectively.

Furthermore, the absence of lysine decarboxylase (LDC) activity in the cured strain strongly implies that the structural genes-encoding LDC in this bacterium is plasmid-encoded.

After curing of plasmid, a 100% of antibiotic-resistance loci were observed as chromosomal encoded.

All of the results shown above indicated that the Desistance is plasmid mediated.

التخصصات الرئيسية

الأحياء

الموضوعات

عدد الصفحات

76

قائمة المحتويات

Table of contents.

Abstract.

Chapter one : Theoretical background.

Chapter two : Literature review.

Chapter three : Design and methodology.

Chapter four : Results and discussion.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Ajrud, Nawal Rashid. (2009). Cadmium-resistant bacteria isolated from Petra city wastewater treatment station. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-304845

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Ajrud, Nawal Rashid. Cadmium-resistant bacteria isolated from Petra city wastewater treatment station. (Master's theses Theses and Dissertations Master). Mutah University. (2009).
https://search.emarefa.net/detail/BIM-304845

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Ajrud, Nawal Rashid. (2009). Cadmium-resistant bacteria isolated from Petra city wastewater treatment station. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-304845

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-304845