Immunohistochemical study of breast carcinoma in old age Iraqi women by application of BRCA1 and P53

الملخص EN

Objectives : to estimate the rate of BRCA1 and P53 immunoexpression in different histological types of female breast cancer and to assess whether these biomarkers are significantly correlated with each other and to assess the expression of these two biomarkers.

To show the correlation of these biomarkers to common clinic pathological parameters such as : tumor grade, size, lymph node involvement, and recurrence of breast carcinoma for old age group.

Methods : Thirty patients with breast carcinoma their ages ranging between 38-60 years with a mean age of 49 years, were included in this study and conducted in the Department of Pathology, Faculty of Medicine, Kufa University from November 2010 through September 2011.

Labeled Streptavidin -Biotin (LSAB+) method was employed for immunohistochemical detection of BRCA1 and p53. Results : 28 cases (93.4 %) were of ductal carcinoma while2 cases (6.6 %) were of lobular type.

The detection rate of BRCA1 and p53 was 92.9 % and 50 % respectively.

Conclusion : there was significant difference of BRCA1 overexpression between ductal and lobular carcinoma, while there was no significant difference of BRCA1 overexpression with different tumor sizes, lymph nodal involvement, age of the patients, and recurrence of breast carcinoma..

P53 immunoexpression was not significantly different among tumor sizes, while there wasa significant difference with axillary lymph node involvement, tumor grade, and recurrence of breast cancer.

However, p53 positively correlated with tumor grade.

Moreover, both biomarkers were positively correlated with each other, and cases expressing both BRCA1 and p53 have shown the most unfavorable bio pathological profile.