Biodegradation of substituted chlorobenzoic acid by bacterial isolates : effect of some growth conditions

العناوين الأخرى

التحطيم الحيوي لمركبات الكلوربنزويك بواسطة عزلات بكتيرية : تأثير ظروف النمو على التحطيم الحيوي

مقدم أطروحة جامعية

al-Masaid, Ata Allah Salman

مشرف أطروحة جامعية

al-Tarawinah, Khalid Ahmad

أعضاء اللجنة

Jaran, Adnan S.
Irshaid, Fawzi
Khalifat, Khalid Muhammad

الجامعة

جامعة آل البيت

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2009

الملخص الإنجليزي

Bacterium Proteus vulgaris was isolated from the wastewater treatment plant of As-Samra Wastewater Treatment Plant which is located in northern Jordan.

It was identified by morphological and biochemical characteristics.

Proteus vulgaris was found to use the Chlorobenzoates compounds as alternative carbon and energy source.

The percentages of degradation of the chlorobenzoates (2-Chlorobenzoate, 3-Chlorobenzoate, 4-Chlorobenzoate and 3,4-Dchlorobenzoate ) at the concentration of 2-mM were found (after 80hr of incubation) 91.4%, 72.75%, 71.4% and 73.2% respectively.

At the 3mM concentration the percentages were 87.33%, 62.1%, 53.8% and 62.5% respectively.

The initial rates were found different according to the substrates and their concentration.

The biodegradation ability was monitored through the release of chloride, disappearance of the substrates and finally the growth of Proteus vulgaris cells on that substrates.

The best result of biodegradation of 2-CBA compound was obtained with 2mM substrate concentration, at 37ºC, pH 8 and 200µl inoculums size.

Adaptation of the cells on 2-CBA for 48 hours and cells starvation for 24 hours and 48 hours increased significantly the initial degradation.

The carbon sources increased the cell biomass.

However, all carbon sources used reduced the biodegradation and Cl production from 2-CBA.

All yeast extract concentration enhanced the growth and reduced the biodegradation percentage as well as the Cl production.

Nitrogen sources reduced the biodegradation activity of the 2-CBA as well as the chlorine release from it.

2-CBA at 2mM was used in further experiments because the 2-CBA is the most toxic one among these compounds.

The experiments were included substrate concentration, temperature, pH, inoculums size, carbon starvation, carbon adaptation, carbon and nitrogen sources.

The preferential concentration, temperature and pH for the strain were 2mM, 37ºC and respectively

التخصصات الرئيسية

الأحياء

الموضوعات

• البكتيريا

عدد الصفحات

80

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-307726