Partial purification and cytotoxic activity of l-asparaginase isolated from escherichia coli

الملخص EN

L-Asparaginase (E.

C.

3.

5.

1.1) is an important natural product that possesses a broad spectrum of antitumor activity.

In the present study, L-asparaginase partially purified from local isolate of Escherichia coli that were grown aerobically for four hours and anaerobically for 18hrs on M9 medium contain L-asparagine as sole nitrogen source.

Extraction of the enzyme was done by sonication.

Qualitative and quantitative assays for L-Asparaginase production were determined using colorimetric and nesslerization methods respectively.

The purification steps involve dialysis of crude extract and DEAE-Cellulose Ion exchange chromatography.

The enzyme was purified 3.39-folds and showed a final specific activity of 0.18 U / mg with a 41 % yield.

The crude extract and DEAE-Cellulose fractions showed slight growth inhibition against RD cell line (human rhambdomyo sarcoma).