التوصيف الجزيئي لبعض أصول الكرمة المنتشرة في سورية باستخدام المؤشرات الجزيئية SSR

الملخص EN

Phylloxera resistance grape rootstocks are widely distributed in nurseries and vineyards in Syria.

The objectives of this study were to characterize and determine the genetic variation in six of these rootstocks (B41, Ruggeri140, So4, Richter99, Fercal, EM333) by using the SSR marker technique (simple sequence repeats).

It has been found that 65 bands (alleles) were determined by using 20 SSR primers, and the proportion of the unique one which appeared only in one grape rootstock was 38.5%.

In addition, it has been noticed that 85% from the studied SSR markers (determine microsatellite loci by using specific primers) demonstrate the polymorphism which can be distinguished between the studied varieties and show the genetic variation obviously.

It was clearly seen by calculating (PIC) coefficient that the greatest ability of demonstrating the variation between the six rootstocks was, by using the primers, (VRZAG62 and VVMD32) where their coefficient value were relatively high (0.78).

According to the cluster analysis and the determination of the relationship , all the studied varieties can be divided into two groups.

The first one contains the rootstocks (B41, Fercal and EM333), whereas the second one includes (Ruggeri140, So4 and Richter99).

The distribution of grape rootstocks into groups and the variation between groups was associated with the distribution of some alleles especially at four microsatellites loci 180,188 at VVS4, 222 at VRZAG62, 148 at Vmc8A7, and 194,214 at VVMD27.