Protection properties of He-Ne laser (632.8 nm)‎ against UV-light (253.7 nm)‎ on the lymphocytes blood cellsand its DNA

الملخص EN

Background : he-Ne laser (632.8 nm) pre-irradiation may lead to modulate the damaging effects of ionizing radiation and decreasing in radiation damage on cells, by the induction of antioxidant defence mechanisms and accelerated the repair.

Objective : to study the protection properties of He-Ne laser (632.8 nm) pre-irradiation against UV light (253.7 nm) damage on human lymphocyte blood cells and its DNA.

Subject & Methods : 27 samples were processed only for lymphocyte blood cells separation, while a 45 samples for the extracted DNA from lymphocyte.

The total of 72 samples were employed to evaluate the influence of He-Ne laser pre- irradiation against UV-light.

Results : the results showed that He-Ne laser (632.8 nm) pre-irradiation on human lymphocyte blood cells and its DNA leads to a decrease in the damage which caused by UV light (253.7 nm) irradiation.

Conclusion : the magnitude of protection depends on the He-Ne laser pre-exposure doses.

A higher survival percentage of lymphocyte blood cells and its DNA occurred with low He-Ne laser dose.