Photodynamic therapy for leiomyosarcoma : in vitro study

الملخص EN

Leiomyosarcoma (smooth muscle tumor) is a rare type of a malignant neoplasm of smooth muscle.

It is a resistant cancer, generally not very responsive to chemotherapy, radiation and surgery.

The aim of this study was to evaluate photodynamic therapy (PDT) as a possible basis for treatment using (650 nm) diode laser with methylene blue (MB) as a photosensitizer on Leiomyosarcoma (LMS) cell line (L20B) in vitro.

The cytotoxic effects of 650nm diode laser 0.052 W/cm2 (power density) at different exposure time (10, 20, and 30 min) with methylene blue at different concentrations (0.01 %, 0.1 %, 1%) was investigated on LMS cell line (L20 B).

The cytotoxic effect of laser was increased with increasing irradiation time for a certain concentration of methylene blue.

The exposure time 30 min of diode laser with 1 % (MB) has the great inhibitory rate (IR) for LMS cells growth which reaches to 80.46 % and reducing the proliferation rate (PR) which reaches to 19.54 %.