In vitro study of methotrexate- polypeptide effect on leishmania donovani replication rates

الملخص EN

(MTX) has been supported by the polypeptide (insulin).

The degree of MTX incorporation into Methotrexate polypeptide (1:0.5) the molar substitution ratio was applied in the experimental reaction.

The spectrophotometers were used for characterization of MTX-polypeptide product.

The in vitro couplings of MTX-polypeptide are shown to be an inhibitant of L.donavani growth.

The growth rate of parasites decreased in parallel with increasing the concentration of MTX- polypeptide (0.02, 0.04 and 0.06 mg / L) & lowering as compared to the control group.

The growth rate is counted during period of as long as 72 hrs. The maximum decline in growth rate was obtained in the third day at concentration of 0.06 mg / L of MTXpolypeptide.

There is a significant difference (p < 0.05) in replication rates of promastigotes among the different concentrations of MTX- polypeptide (mainly at 0.06 mg / L).