Study of some essential requirements of vitamins for in vitro micropropagation of date palm (Phoenix dactylifera L.)‎ cv. barhee

الملخص EN

This study was conducted to investigate the effects of vitamins: Biotin, Pyridoxine, CPantothenate, and Riboflavin on the embryogenic callus and somatic embryos of date palm (Phoenix dactylifera L.) CV.

Barhee. The results showed that the best significant effect at level 2 mg / L for each vitamin on embryogenic callus was 4.9, 4.6, 4, 3.9 gm.

Respectively, whereas the less values at control treatment were 3.2, 3.6, 2.9, 3 gm respectively. There are significant values for the maturation period of the somatic embryos at level 2mg / L for the four vitamins were 25, 26, 26, 29 day respectively as compared with control treatment. The best significant effect for interaction between biotin and pyridoxine on the number of somatic embryos at level 2 mg / L for both was 28 embryos whereas for interaction between pantothenate and riboflavin also at 2mg / L for both was 27.6 embryos and the less values are at control treatment ; Finally the best significant effect for interaction between biotin and pyridoxine on the fresh weight of somatic embryos at level 2mg / L for both was 0.280 whereas the less values at control treatment were 0.086, 0.084 gm respectively.