The physiohistological effect of pesticide endosulfan 35 % on sexual hormone on female rabbits and its role in environmental pollution in Marsh area South of Iraq

الملخص EN

Endosulfan is an insecticide which used in illegal& misused application by fisherman as a mean of fish hunting purpose in marsh area / south of Iraq.

This study was undertaken to investigate the effect of oral administration of three different doses of Endosulfan 35 % on the sexual hormone level (LH, FSH & Estradiol) in addition to histological study of uterus, ovaries on female rabbits and study their effect on environment pollution.

48 mature female rabbits were randomly divided into 4 groups of equal number (12) on each as follow : first group which administered 0.9 ml NACL which act as control, the second, third , fourth groups which administered Endosulfan 35 % as concentration( 0.5ml ,1ml,1.5 ml / kg .B.W ) respectively.

After 8 weeks, the one half of animals of each groups (6 rabbits) were sacrificed, the blood sample was taken for hormonal assay (LH, FSH & Estradiol) and histological examination of uterus, ovaries were done.

After recovery period (5 weeks) the same parameters mention above was measured.

The results showed that Endosulfan35 % regarded as persistent organic pollutant caused endocrine disruptor and causing lowering of LH, FSH & Estradiol level and did not return to normal value after recovery period.

Histological changes were noticed in uterus including fibrotic polyp (tumor like) in fourth group (1.5 ml), as well as reduce endometerium in all treated groups as compared with control group.

Whereas the ovaries showed primary and secondary follicles in third and fourth groups (1ml, 1.5ml) and did not return to normal structure after recovery period.