Effects of gold and silver colloidal on gama glutamate transferase enzyme activity in blood serum

الملخص EN

Laser ablation of metal plate in water was performed in order to obtain gold and silver nanoparticles.

Transmittance electron microscopy TEM and uv-vis spectrophotometer were conducted in order to determine the size and optical properties of the nanoparticles, respectively.

The nanoparticles concentrations were also characterized by atomic absorption spectroscopy AAS measurement.

The absorbance spectra show a sharp and peaks around 400 or 525 nm, indicating the production of silver and gold nanoparticles with average size of 25 nm, have been confirmed by TEM.

Both Gold and silver nanoparticles show inhibition on the Gama glutamate transferase (γ-GT) activity.

The inhibition effect increase as a function of nanoparticles concentrations.

Kinetic properties of (γ-GT) activity revealed (by nanoparticles) mixed type of inhibition.