Indirect spectrophotometric method for the determination of bisacodyl in commercial dosage forms and in environmental water samples

الملخص EN

Objective : A simple, sensitive, and accurate indirect spectrophotometric method for the determination of bisacodyl in pure form and in some of its pharmaceutical preparations.

Method : The method is based on the oxidation of bisacodyl by iron (III), and subsequent complexation of iron (II) with o-phenanthroline.

Results: Forming a red-colored complex (ferroin) having the maximum absorbance at 510 nm.

Beer’s law is obeyed in the concentration range of 0.5-5 μg / ml.

The molar absorptivity and sandell’s sensitivity were 1.55 × 104 L.mol-1.cm-1 and 0.0233μg.cm-2 respectively.

The relative standard deviation (RSD) was less than 1.5 (n = 11).

The limits of detection and quantitation are 0.083 and 0.25 μg.ml-1 respectively.

Conclusion : The method is applied successfully for determination of bisacodyl in environmental water samples and in some pharmaceutical formulations (tablets and suppositories).

A statistical comparison of these results with those of official method shows good agreement and indicates no significant difference in the precision.