Effect of yeast extract on biodegradation of 3-chlorobenzoic acid by corynebacterium auris

العناوين الأخرى

تستعمل البكتيرياCorynebacterium auris و المعتمدة علي 3-CBA كمصدر كربوني وحيد

مقدم أطروحة جامعية

al-Allawi, Saddam Mustafa

مشرف أطروحة جامعية

al-Tarawinah, Khalid Ahmad Jafar

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2012

الملخص الإنجليزي

Corynebacterium auris bacteria were found to use 3-CBA as a sole source of carbon.

The biodegradation ability was monitored through the disappearance of the substrate, the release of chloride and the growth of bacterial cells.

The highest percentage of biodegradation at the standard conditions with 2 mM substrate concentration is 80 % after 80h of incubation.

However, the percentage of degradation increased to 85 % at the adaptation conditions using 2mM 3-CBA concentration in the growth medium.

Adaptation of the cells on 3-CBA for 48 hours and cells starvation for 48 hours increased significantly the initial degradation rate.

The percentage of degradation of 3-CBA by Corynebacterium auris varied enormously according to the conditions of culture and the substrate concentration in the medium.

The initial rates of biodegradation ranged from 35 to 60 µM / h according to different growth conditions at the 2 mM substrate concentration. The two carbon sources used in this study enhanced the biodegradation.

The percentage of degradation of 2mM 3-CBA by Corynebacterium auris using glucose and sucrose as second carbon source in the medium were 84% and 83% respectively.

The chloride release was in accordance with the biodegradation of 3-CBA in all the experiments.

However, the nitrogen sources yeast extract and tryptone decreased the degradation when compared to that with the substrate only in the minimal salt medium.

The percentage of degradation using yeast extract and tryptone were 60 % and 50 % respectively.

The experiments included substrate concentration, temperature, pH, carbon starvation, carbon adaptation ; carbon and nitrogen sources .The optimal concentration, temperature and pH for the strain were 2mM, 37ºC and PH 7 respectively.

التخصصات الرئيسية

الأحياء

الموضوعات

• التكنولوجيا الحيوية

عدد الصفحات

60

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-322624