Isolation, identification and biotyping of Brucella Spp. from milk product at Basrah Province

الملخص EN

Three hundred milk product samples were collected from different locations of Basrah province.

By using enrichment broth technique and Brucella selective medium nine isolates (8 from soft cheese and one from cream) where isolated.

No Brucella strain was isolated from ice-cream.

The species and biotypes of these isolates were determined and it was found that 4 isolates of Brucella abortus biotype 4 and 5 isolates of B.

melitensis biotype 2.

Antibiotic sensitivity test showed that all isolates were sensitive to streptomycin, gentamycin, kanamycin, rifampicin, trimethoprim, trimethoprim with sulfamethoxazole and tetracycline, some strains showed resistance to doxycyclin, cephalexin, ampicillin and erythromycin.

Brucella isolates also showed growth in different range of temperature and pH.