Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women : a comparative analysis of their cytotoxic effects with commercial vaginal probiotics
المؤلفون المشاركون
Mutivasili, Ilahi
Shirzad, Mahdi
Raufian, Riza
Hashiminasab, Sayyid Muhammad
Hatami, Maryam
Dianatpur, Mahdi
Mudarrissi, Muhammad Husayn
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 15، العدد 3 (31 مارس/آذار 2013)، ص ص. 199-206، 8ص.
الناشر
تاريخ النشر
2013-03-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : Vaginal flora of healthy women is dominated by Lactobacillus species which can prevent bacterial vaginosis.
Objectives : The current study aimed to determine the differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis (BV) infected women and compared their cytotoxic effects with commercial vaginal probiotics.
Patients and Methods : One hundred and seventy eight vaginal specimens were collected from healthy and BV infected women.
Lactobacillus colonies were obtained by culturing on laked blood BHI and MRS medias and genetically defined by 16s rRNA sequencing.
Differentiating the specimens to normal, intermediate and BV infected were carried out by Ison and Hey grading protocol.
Identification of Lactobacillus strains in vaginal specimens were performed by Multiplex PCR.
The inhibitory effects of lactobacilli on Hela (tumoral cervical cells) and HNCF-pi52 (normal cervical cells) were conducted by MTT and trypan blue assays.
Results : L.
crispatus, L.
gasseri, L.
iners, L.
jensenii, L.
acidophilus and L.
rhamnosus were the most frequently occurring species in vagina of healthy Iranian women.
L.
crispatus and L.
jensenni were significantly higher in the normal than in the BV infected groups.
Also the cytotoxic effect of L.
crispatus on tumoral cervical cells was higher than other lactobacilli including commercial probiotics.
Conclusions : As L.
crispatus and L.
jensenni were significantly higher in BV infected women and the cytotoxic effect of L.
crispatus on tumoral cervical cells was high, introduction of new probiotics seems necessary.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mutivasili, Ilahi& Shirzad, Mahdi& Raufian, Riza& Hashiminasab, Sayyid Muhammad& Hatami, Maryam& Dianatpur, Mahdi…[et al.]. 2013. Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women : a comparative analysis of their cytotoxic effects with commercial vaginal probiotics. Iranian Red Crescent Medical Journal،Vol. 15, no. 3, pp.199-206.
https://search.emarefa.net/detail/BIM-329085
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mutivasili, Ilahi…[et al.]. Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women : a comparative analysis of their cytotoxic effects with commercial vaginal probiotics. Iranian Red Crescent Medical Journal Vol. 15, no. 3 (2013), pp.199-206.
https://search.emarefa.net/detail/BIM-329085
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mutivasili, Ilahi& Shirzad, Mahdi& Raufian, Riza& Hashiminasab, Sayyid Muhammad& Hatami, Maryam& Dianatpur, Mahdi…[et al.]. Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women : a comparative analysis of their cytotoxic effects with commercial vaginal probiotics. Iranian Red Crescent Medical Journal. 2013. Vol. 15, no. 3, pp.199-206.
https://search.emarefa.net/detail/BIM-329085
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 205-206
رقم السجل
BIM-329085
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر