Histochemical and immunohistochemical techniques in ulcerative colitis

الملخص EN

Objective : To demonstrate the mucin changes in Ulcerative Colitis, to evaluate the benefit of the staining method and to demonstrate the CEA staining pattern in dysplasia complicating ulcerative colitis (U.C).

Methods : Colorectal biopsies were examined for changes of U.C for which a combined PAS / Alcian blue stain was applied.

CEA immunohistochemical stain was used for cases of dysplasia complicating ulcerative colitis.

Results : One hundred colorectal biopsies were examined, U.C.

was diagnosed in (22 %) of cases, the mean age was (36.68) years, (13) were males, and (9) were females.

The prevalence of dysplastic changes complicating U.C were detected in (22.7 %).The rectum was the most frequent site of dysplasia complicating, U.C..

Two of the cases showed mild dysplastic changes ; two showed moderate dysplasia, and only one showed severe dysplasia.

The secretory activity of mucin in colorectal mucosa was tow in surface epithelium and varied in the crypts from absent or weak to moderate reduction.

The site of CEA distribution seemed to be affected mainly by degree of dysplasia, it was predominantly along the apical surface of the cells and also in the cytoplasm in case of mild dysplasia, while in moderate dysplasia it was of cytoplasmic distribution, and in severe dysplasia there was intensive cytoplasmic distribution.

Conclusion : There was an increase in relative frequency of U.C.

Immunohistochemical study of CEA localization in dysplastic gland is helpful in detection of early malignant change in U.C.