تنقية الكارنوسين من عضلة صدر الدجاج و تشخيصه ببعض تقنيات الكروماتوكرافيا

الملخص EN

Carnosine from chicken pectoral muscle was extracted using water and purified by ion –exchange chromatography using CM-cellulose.

Carnosine was identified by paper chromatography , high-performance liquid chromatography (HPLC) and ultraviolet spectrophotometer.the yield of extracted and purified carnosine was 100 mg / 100g of chicken pectoral muscle.Paper chromatography showed high purity of carnosine after ion-exchange purification and had Rf ×100 value of 20.4 .Two spots were obtained on paper chromatography after the treatment of the resultant purified carnosine with 6 N hydrochloric acid representing β-alanine and histidine which compose carnosine .These spots had Rf×100 values of 40.1, 16.7 respectively.

One peak representing carnosine with retention time 6.4 min was obtained by HPLC.

Two peaks were obtained on HPLC after treatment of carnosine with 6N hydrochloric acid, with retention time of 3.435, 4.423, respectively, which were close to retention times of the standard amino acid β-alanine and histidine 3.525, 4.503 min, respectively.

Carnosine exhibited highest absorption at 212 nm as detected UV-spectrophotometericelly