Studying the resistant capacity of wild type and mutant type of bacillus brevis bacteria

الملخص EN

N-methyl-n-nitro-n-nitrosoguanidin (MNNG) and acridin orange (A.O.) was used as mutagenic agents for generation of gramicidin negative mutants of Bacillus brevis.

Three gramicidin negative mutants were obtained by using MNNG (1000mg\ml) and ten gramicidin negative mutants were obtained by using A.O.

(3mg \ ml).

A relationship between streptomycin resistance and suppression of gramicidin production was noticed.

The resistance to streptomycin was increased five folds in mutant strain as compared to the wild strain.