دراسة الظروف المثلى لإنتاج المواد الحيوية ذات النشاط السطحي (الرامنولبيد)‎ من العزلة المحلية Pseudomonas aeruginosa لبكتريا P. a. 28

الملخص EN

Thirty five isolates of Pseudomonas were isolated from different sources, only 29 isolates were identified as P.

aeruginosa, the ability of isolates were tested for production of biosurfactant (Rhamnolipid) using sunflower oil as carbon source and wheat bran as nitrogen source.

The isolates P.

a.

28 which was isolated of petroleum well of Nehran Omer was selected for its high production of biosurfactant.

The optimal conditions for the production of rahmnolipid by the local isolate P.a.

28 were studied which represented by a media consist of 4% sunflower oil as a carbon source, 0.6 % wheat bran as a nitrogen source with pH 7, 1% volume of inoculum and the incubation temperature was 30 ° C using shaking incubator at 180 rpm for 60 hours.

The maximum production of rhamnolipid was (28.9 g/l).

Rhamnolipid was identified by using thin-layer chromatography, The results observed that this isolate prodused two kinds of monorhmnolipid and dirhamnolipid.