High performance liquid chromatographic method for determination of sildenafil citrate (viagra)‎ in pharmaceutical formulation and industrial effluent sample

الملخص EN

A simple, precise, rapid and accurate reversed–phase high performance liquid chromatographic method has been developed for the determination of sildenafil citrate in a pure form, pharmaceutical formulations and industrial effluent samples.

Chromatography was carried out on supelco L7 reversed- phase column (25cm × 4.6mm), 5 microns, using a mixture of methanol : water : triethylamine (630 : 370 : 2 v / v / v) and adjusted to pH 4.0 with dilute phosphoric acid as a mobile phase at a flow rate of 1.0 ml.min-1.Uv detection was performed at 220 nm at ambient temperature (25 °C).

The retention time for sildenafil citrate found to be 4.6 minutes.

The calibration curve was linear (r = 0.9998) over a concentration range from 10 to 70 Μg / ml.

Limit of detection (LOD) and limit of quantification (LOQ) were 60 ng / ml and 180 Ng / ml respectively.

The method was validated for its linearity, precision and accuracy.

The proposed method was successfully applied to the determination of sildenafil citrate in tablets and industrial effluent samples.