Some immunological and biochemical aspects for hepatitis B virus detection

الملخص EN

The Study Was Carried Out On 1200 Persons Who Suspected To Be Hepatitis B Virus Infected, Admitted To AL-Hilla Teaching Hospital / Central Public Health Laboratory, To Investigate Some Sero- Immunological And Biochemical Tests.

Biochemical Tests Of Liver Function Enzymes Revealed Enhancement In Both Alanine Aminotransferase (ALT) As Well As Aspartate Aminotransferase (AST) Levels In All Patient Sera In Comparison With Normal Healthy Control Group, However Both Enzymes Appeared To Be High In Male Than Female The Highest Mean Value For (ALT) Of Infected Subject Occur In The Male Within Age Group (51 60) Years Old.

Which Reach To 608.8 U / L.

While The Highest Mean Value For (ALT) In Female Occur Within Age Group (31 40 )Years Old, Which Reach To 380.3U / L.

While The Highest Mean Value For (AST) In Male Occur In Age Group (51–60) Years Old Which Reach To 532.2U / L, Whereas The Highest Mean Value For (AST) In Female Occur In Age Group(41–50) Years Old Which Reach To 367.2 U / L.

ELISA Assay For Detection Of Hepatitis B Virus Surface Antigen As Well As Igm Anti Hepatitis B-Core Antigen And Anti-Hbeag Were Used In This Study For Detection Of HBV And Its Immunological Response In Infected Subjects .

The Result Revealed That Only 48 Out Of 1200 Patients Had Hbsag Positive (4 %) With High Frequency In Male (2.1 %), Than Female(1.3 %) Group.

On The Other Hand.

The Highest Percentage Of Igm-Anti Hbcag Was Detected Within Age Group (31 40) Years Old Which Reach To 77.7 % Whereas, Anti-Hbeag Reach Its High Percentage Within Age Group (51 60) Years Old Which Reach To 15.3 %.

This Study Concluded That Males Were More Susceptible To Infection With HBV Than Females And Chronisity Of The Disease Was Directly Associated With Age Increase And Recommended That Vaccination Program Must Be Carried Out For Young Population To Perform Protection Early In Life, To Prevented.

Transmission of the Viruses Through Population, With Continuous Use Of Screening Test As ELISA And PCR For Monitoring Of The Virus.