Effect of short-term flushing with lupin grain during pre-ovulatory period on ovarian activity and metabolic changes in Damascus female goats

الملخص EN

The present study was designed to evaluate the effects of short-term flushing using lupin grain (high energy-protein diet) during pre-ovulatory period on ovarian activity and metabolic changes in Damascus female goats.

Estrous cycle was synchronized in Sixteen Damascus does using CIDR for 6 days plus an intramuscular injection of prostaglandin F2α (PGF2α) at CIDR removal.

Half of the does received the maintenance diet (control, C-group), while the second half received the maintenance diet plus supplementation of 500 g of lupin grain (F-group).

Feeding treatment lasted six days (from CIDR insertion to CIDR removal).

The flushed does had higher mean number of ovulatory (≥ 5mm) follicles during the follicular phase (P < 0.05), and higher ovulation rate during the subsequent luteal phase (P < 0.05).

The nutritional treatment increased serum concentrations of glucose (P < 0.001) and urea (P < 0.001) during the pre-ovulatory period.

Serum insulin concentration tended to be higher in the F-group than in the C-group on day 2 of nutritional treatment and reached the significant value on the day 4 of nutritional treatment.

Serum concentrations of insulin- like growth factor (IGF-I) did not differ between both groups during all experimental period (P > 0.05).

A positive correlation was observed between concentration of serum glucose and both of number of ovulatory follicles during the follicular phase and ovulation rate, while negative correlation (P=0.06) was observed between serum urea concentration and serum progesterone concentration on the subsequent luteal phase.

Results suggest that short-term flushing using high energy-protein diet during pre-ovulatory period enhance ovulation rate in Damascus female goats.

This effect seems to be related with high concentration of serum glucose and insulin during pre-ovulatory period.

Contrary, elevation of undesirable metabolites as urea (when high protein diets are used) may impair the final goal of flushing due to the negative effects on corpus luteum function.