Comparative analysis of genes related to quality of oocyte in buffaloes (Bubalus bubalis)‎ and camels (Camelus dromedarius)‎ under in vitro conditions

الملخص EN

The quality of oocyte is one of the main determinants of in vitro embryo production success.

Different approaches have been applied for analyzing oocyte qualities particularly gene expression analysis, which represents a new tool for selecting competent ones.

Thus, the present study aimed at studying gene expression of morphologically good and poor immature oocytes of buffaloes and camels.

A total of 360 COCs of buffaloes and camels (n = 180 each) were selected to run this study.

Out of which 90 were pooled in three replicates with 30 oocytes in each of the two groups (immature good and poor grade oocytes) were used for gene expression analysis after cumulus cells denudation.

Total RNA was isolated from all samples using PicoPure TM RNA isolation kit, and then converted into cDNA via reverse transcription kit.

Quantitative Real-Time PCR was performed using specific primers for a set of selected candidate genes regulating cell cycle (Cyclin B1 and PTTG1), transcription factor activity (OCT4), polyamine biosynthetic (ODC1), signal transduction and activation of transcription (STAT3).

Quantitative real-time PCR results showed a higher expression of all the selected genes in good compared to poor grade oocytes except of STAT3 which showed a similar expression in camels' oocytes.