Effect of chitosan sheet on inflammation in rabbits

الملخص EN

This study was conducted to investigate the effect of chitosan sheets on A inflammation.

Accordingly chitosan sheet was isolated and prepared from the exoskeleton of the native shrimps in Basrah Province.

The inflammation was induced and evaluated by polyvinyl sponge to determine the events which occur during the inflammatory process during different intervals in the presence or absence of chitosan ‘ sheets .For this purpose 24 male rabbits were used and two linear skin incisions (2 cm) were made on the dorsal aspect in both sides of each rabbit.

The inflammatory process at l, 3, & l5 day post wounding was evaluated macroscopically in terms of hyperemia, suppuration, dryness, and adhesion of margins and microscopically in terms of infiltration of inflammatory cells specially neutrophils and macrophages, fibrovascular granulation tissue.

The ‘results of macroscopic evaluation revealed that '-It first post wounding day the test wound was significantly (p < 0.

05) more hyperemic than control wounds followed by a significant decrease in the severity of hyperemia in test wounds at ls‘, 3rd, 7"‘, and 15"‘ post wounding days (p < 0.

05).

The test wounds was more dry with less suppuration to-words the end of the experiment than control wounds (p < 0.

05).

The adhesive contact between the margin of the wounds was significantly higher in test wounds than in control wounds through out the period of experiment (p < 0.

05).EFFECT OF CHITOSAN SHEET ON INFLAMMATION IN RABBITS A Ala Al-Deen H.

J awad*, J asim M.

Al-Diab** and Manal Kh.Ibraheem*** *Department of physiology,College of Veterinary Medicine, University of Basrah,Basrah,Iraq.

**Department of pathology,College of Medicine, University of Basrah,Basrah,Iraq.

‘ ' ***College of Pharmacy, University of Basrah, Basrah, lraq.

‘ (Received 22 November 2005, Accepted 13 January 2006) Kcywords;glnflammation,Chitosan,hyperemic.

.

ABSTRACT This study was conducted to investigate the effect of chitosan sheets on A inflammation.

Accordingly chitosan sheet was isolated and prepared from the exoskeleton of the native shrimps in Basrah Province.

The inflammation was induced and evaluated by polyvinyl sponge to determine the events which occur during the inflammatory process during different intervals in the presence or absence of chitosan ‘ sheets .For this purpose 24 male rabbits were used and two linear skin incisions (2 cm) were made on the dorsal aspect in both sides of each rabbit.

The inflammatory process at l, 3, 7 & l5 day post wounding was evaluated macroscopically in terms of hyperemia, suppuration, dryness, and adhesion of margins and microscopically in terms of infiltration of inflammatory cells specially neutrophils and macrophages, fibrovascular granulation tissue.

The ‘results of macroscopic evaluation revealed that '-It first post wounding day the test wound was significantly (p < 0.

05) more hyperemic than control wounds followed by a significant decrease in the severity of hyperemia in test wounds at ls‘, 3rd, 7"‘, and 15"‘ post wounding days (p < 0.

05).

The test wounds was more dry with less suppuration to-words the end of the experiment than control wounds (p < 0.

05).

The adhesive contact between the margin of the wounds was significantly higher in test wounds than in control wounds through out the period of experiment (p < 0.

05).