Study of the biological activity of compounds isolated from lawsonia inermis

الملخص EN

This research work was aimed to separate some compounds that have a biological activity from Lawsonia inermis leaves.

The plant leaves were obtained from southern part of Iraq-Basrah-Al-Faw as a semidry.

The biological activity was examined on both Gram positive and negative bacteria.

Pseudomonas aeruginosa, Escherichia Coli, Proteus mirabilis, Salmonella typhi, Klebsiella pneumoniae, Bacillus subtilis and Staphylococcus aureus.

The study involve extraction of some tanny materials employing ethanol.

Ethanolic extract was fractioned over silica gel column (80-120) mesh.

Fractions isolated were eluated by chloroform, acetone and finally ethanol.

The separated fractions were further analyzed by IR, TLC plate and chemical tests.

The results revealed the chloroform extract contains mainly p-hydroxy benzoic acid, and the acetone extract contains mainly caffeic acid while the ethanolic fractions contains tannic acid.

The chemical structure of each analyzed compounds was concluded from comparison with that reported in the literature.

Application of p-hydroxy benzoic acid to both Gram positive and negative bacteria indicated that all types of bacteria were affected with some difference except Pseudomonas aeruginosa.

Caffeic acid fluctuated on the bacteria Salmonella typhi, Escherichia Coli, Bacillus subtilis and Staphylococcus aureus but not on Pseudomonas aeruginosa, Proteus mirabilis, Klebsilla pneumonia.

However, extract include tannic acid showed agreed effect on all types of bacteria under study when it compared to effect of extract include p-hydroxy benzoic acid, caffeic acid and the control (Gentamycin and Tetracycline).