![](/images/graphics-bg.png)
Preparation of C-reactive protein latex reagent of human serum
العناوين الأخرى
تحضير محلول لاتكس لبروتي Cالمنفعل من مصل الإنسان
المؤلفون المشاركون
al-Shalji, Sahar Abd al-Wahhab
Mahdi, Layla Khalid
Sabbar, Muna
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 5، العدد 1 (30 يونيو/حزيران 2006)، ص ص. 112-122، 11ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2006-06-30
دولة النشر
العراق
عدد الصفحات
11
التخصصات الرئيسية
الموضوعات
الملخص AR
C-reactive protein (CRP) is an acute phase reactant the concentration of which increases significantly following tissue injury or inflammation.
It is present in the albumin fraction of the serum of patients and is antigenic upon injection into rabbits.
We report here the development of a local latex reagent coated with rabbit anti-CRP.
For this purpose CRP was isolated from pooled sera of patients and injected into rabbits.
CRP rabbit antiserum was purified by using ammonium sulfate precipitation.
Followed by passing on ion exchange chromatography (Diethylaminoethyl "DEAE" cellulose).
Then, latex particles were coated with purified antisera.
The prepared CRP latex reagent agglutination was observed upon the addition of patients' sera and compared with that of normal sera.
In addition, it was evaluated against a standard CRP latex reagent.
The prepared reagent gave positive results (100%) with patients' sera and the positive control of the standard kit.
While, the negative control of the standard kit and 30% of the normal sera gave negative results.
الملخص EN
C-reactive protein (CRP) is an acute phase reactant the concentration of which increases significantly following tissue injury or inflammation.
It is present in the albumin fraction of the serum of patients and is antigenic upon injection into rabbits.
We report here the development of a local latex reagent coated with rabbit anti-CRP.
For this purpose CRP was isolated from pooled sera of patients and injected into rabbits.
CRP rabbit antiserum was purified by using ammonium sulfate precipitation.
Followed by passing on ion exchange chromatography (Diethylaminoethyl "DEAE" cellulose).
Then, latex particles were coated with purified antisera.
The prepared CRP latex reagent agglutination was observed upon the addition of patients' sera and compared with that of normal sera.
In addition, it was evaluated against a standard CRP latex reagent.
The prepared reagent gave positive results (100 %) with patients' sera and the positive control of the standard kit.
While, the negative control of the standard kit and 30 % of the normal sera gave negative results.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shalji, Sahar Abd al-Wahhab& Mahdi, Layla Khalid& Sabbar, Muna. 2006. Preparation of C-reactive protein latex reagent of human serum. Iraqi Journal of Biotechnology،Vol. 5, no. 1, pp.112-122.
https://search.emarefa.net/detail/BIM-350459
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shalji, Sahar Abd al-Wahhab…[et al.]. Preparation of C-reactive protein latex reagent of human serum. Iraqi Journal of Biotechnology Vol. 5, no. 1 (Jun. 2006), pp.112-122.
https://search.emarefa.net/detail/BIM-350459
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shalji, Sahar Abd al-Wahhab& Mahdi, Layla Khalid& Sabbar, Muna. Preparation of C-reactive protein latex reagent of human serum. Iraqi Journal of Biotechnology. 2006. Vol. 5, no. 1, pp.112-122.
https://search.emarefa.net/detail/BIM-350459
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 121-122
رقم السجل
BIM-350459
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)