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Optimization of phytase production by penicillium purpurogenum GE1 under solid state fermentation by using box–behnken design
المؤلفون المشاركون
Awad, Ghadah E. A.
Hilal, Muhammad M. I.
Daniyal, Inas N.
Isawi, Muna A.
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 21، العدد 1 (31 يناير/كانون الثاني 2014)، ص ص. 81-88، 8ص.
الناشر
تاريخ النشر
2014-01-31
دولة النشر
السعودية
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
Phytase production by Penicillium purpurogenum GE1 isolated from soil around bean root nodules was investigated by solid state fermentation (SSF) using mixed substrates consisted of corn cob and corn bran.
The SSF conditions were optimized by using one-variable–at-a-time strategy.
The optimum conditions for phytase production were at 27 °C, pH 8 and 66 % moisture content.
The study of different carbon and nitrogen sources revealed that glucose and peptone registered the highest enzyme productivity (92 ± 5.6 U / g ds, 125 ± 4.9 U / g ds).
Among different surfactants, maximum phytase productivity was observed with Tween 80 at 0.001 concentrations (170 ± 4.2 U / g ds).
A Box–Behnken design was employed to investigate the optimization of the most significant variables affecting the enzyme production.
Maximal phytase production was detected after the addition of (g / 5 g ds) : 0.75 glucose, 0.375 peptone and 0, 01 tween 80.
This result represented an improvement in phytase production of 2.6 folds when compared to that previously obtained using the basal medium under the same cultivation conditions.
The generated model was found to be very adequate for phytase production (90 % accuracy) as the experimental value was 444 ± 3.5 U / g ds compared to 401 U / g ds for the predicted value.
In brief, the production of phytase using corn cob and corn bran is a novel and cheap way for the production of this important enzyme and opens a new way for researchers to discover and explore this arena.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Awad, Ghadah E. A.& Hilal, Muhammad M. I.& Daniyal, Inas N.& Isawi, Muna A.. 2014. Optimization of phytase production by penicillium purpurogenum GE1 under solid state fermentation by using box–behnken design. Saudi Journal of Biological Sciences،Vol. 21, no. 1, pp.81-88.
https://search.emarefa.net/detail/BIM-351172
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Awad, Ghadah E. A.…[et al.]. Optimization of phytase production by penicillium purpurogenum GE1 under solid state fermentation by using box–behnken design. Saudi Journal of Biological Sciences Vol. 21, no. 1 (Jan. 2014), pp.81-88.
https://search.emarefa.net/detail/BIM-351172
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Awad, Ghadah E. A.& Hilal, Muhammad M. I.& Daniyal, Inas N.& Isawi, Muna A.. Optimization of phytase production by penicillium purpurogenum GE1 under solid state fermentation by using box–behnken design. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 1, pp.81-88.
https://search.emarefa.net/detail/BIM-351172
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 87-88
رقم السجل
BIM-351172
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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