Biochemical studies on the production of sparsomycin antibiotic by Pseudomonas aeurginosa, AZ-SH-B8 using plastic wastes as fermented substrate
المؤلفون المشاركون
المصدر
Journal of Saudi Chemical Society
العدد
المجلد 16، العدد 1 (31 يناير/كانون الثاني 2012)، ص ص. 35-44، 10ص.
الناشر
تاريخ النشر
2012-01-31
دولة النشر
السعودية
عدد الصفحات
10
التخصصات الرئيسية
الموضوعات
الملخص EN
Hundred and twenty microbial isolates could be isolated from different soil samples collected from different localities in Egypt.
One of the bacterial cultures AZ-SH-B8 was found to produce a wide spectrum antimicrobial agent when cultivated on plastic wastes.
The bacterial culture AZ-SH-B8 could be isolated from a soil sample collected from Sharkia governorate, Egypt.
From the taxonomic features, the bacterial isolate AZ-SH-B8 matches with Pseudomonas aeurginosa in the morphological, physiological and biochemical characters and confirmation by using API20E system.
Thus, it was given the suggested name P.
aeurginosa, AZ-SH-B8.
The parameters controllingthe biosynthetic process of antimicrobial agent formation includes: innoculum size, different pH values, different temperatures, different incubation period, different carbon and nitrogen sources and different mineral salts concentrations (KNO3, K2HPO4, MgSO4ئ7H2O and KCl) were fully investigated.
The active metabolite was extracted using n-butanol (1:1, v/v) at pH 7.0.
The separation of the active ingredient and its purification was performed using both thin layer chromatography (TLC) and column chromatography (CC) techniques.
The physico-chemical characteristics of the purified antibiotic viz.
color, melting point, solubility, elemental analysis, spectroscopic characteristics and chemical reactions have been investigated.
This analysis indicates a suggested empirical formula of C13H21N3O6S2.
The minimum inhibition concentrations ‘‘MICs’’ of the purified antimi- crobial agent were also determined.
The purified antimicrobial agent was suggestive of belonging to Sparsomycin antibiotic produced by P.
aeurginosa, AZ-SH-B8.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ata, Husam M.& Radwan, Hasan G.. 2012. Biochemical studies on the production of sparsomycin antibiotic by Pseudomonas aeurginosa, AZ-SH-B8 using plastic wastes as fermented substrate. Journal of Saudi Chemical Society،Vol. 16, no. 1, pp.35-44.
https://search.emarefa.net/detail/BIM-352008
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ata, Husam M.& Radwan, Hasan G.. Biochemical studies on the production of sparsomycin antibiotic by Pseudomonas aeurginosa, AZ-SH-B8 using plastic wastes as fermented substrate. Journal of Saudi Chemical Society Vol. 16, no. 1 (Jan. 2012), pp.35-44.
https://search.emarefa.net/detail/BIM-352008
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ata, Husam M.& Radwan, Hasan G.. Biochemical studies on the production of sparsomycin antibiotic by Pseudomonas aeurginosa, AZ-SH-B8 using plastic wastes as fermented substrate. Journal of Saudi Chemical Society. 2012. Vol. 16, no. 1, pp.35-44.
https://search.emarefa.net/detail/BIM-352008
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 43-44
رقم السجل
BIM-352008
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر