Simultaneous high performance liquid chromatographic (HPLC) determination of ACE inhibitors in pharmaceutical formulations
العناوين الأخرى
الفحص المتزامن للمادة المثبطة HPLC في التحضيرات الصيدلانية بواسطة جهاز ال (ACE-I)
مقدم أطروحة جامعية
مشرف أطروحة جامعية
الجامعة
جامعة عمان الأهلية
الكلية
كلية الصيدلة و العلوم الطبية
القسم الأكاديمي
قسم العلوم الصيدلانية
دولة الجامعة
الأردن
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2013
الملخص الإنجليزي
The purpose of this study was to develop a new high performance liquid chromatographic method for determination of ACE Inhibitors in pharmaceutical formulations.
In the present study a rapid, simple, economical, reverse phase HPLC-PDA method was developed, optimized and validated.
The HPLC method was developed and optimised using Design Expert™ (Version 8.0.6, Stat Ease Inc.
USA) software using central-composite-design, after studying the physicochemical properties.
The Optimised mobile phase was 58 % buffer (0.005M KH2PO4, 0.25ml TEA / L), 25 % acetonitrile and 17 % methanol, final pH was adjusted to 2.80 ± 0.10 using orthophosphoric acid.
The flow rate was 1.0 ml / min.
The signals were monitored using PDA detector (set between λ 200-400 nm).
Quantitation was carried out at 215 nm.
The run time was less than 8 minutes.
The linearity of the developed method was ranged from 5-35 μg / ml for different drugs.
LOD were 0.232, 0.345, 0.476, 0.026, 0.280, 0.609, and 0.141 μg / ml for lisinopril, hydrochlorothiazide (HCT), captopril, imidapril, indapamide, perindopril and trandolapril respectively.
Whereas LOQ were 0.702, 1.044, 1.443, 0.079, 0.848, 1.846 and 0.428 μg / ml for lisinopril, HCT, captopril, imidapril, indapamide, perindopril and trandolapril respectively.
The intra- and inter-day precision (coefficient of variation) of the method was less than 2 %, which indicates highly precise result during the study.
The accuracy of the method were ranged from 102.4-106.6, 99.5-103.5, 102.9-104.3, 97.0¬99.0, 97.6-98.8, 97.0-99.6 and 97.0-98.6 % for lisinopril, HCT, captopril, imidapril, indapamide, perindopril, and trandolapril respectively.
In conclusion this method presented here is sensitive, rapid, accurate, precise, economic, robust, rugged and selective for the routine analysis of the drug in formulation and raw material without changing the chromatographic parameters for these drugs.
التخصصات الرئيسية
الموضوعات
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dawud, Iyad Rashid. (2013). Simultaneous high performance liquid chromatographic (HPLC) determination of ACE inhibitors in pharmaceutical formulations. (Master's theses Theses and Dissertations Master). Al-Ahliyya Amman University, Jordan
https://search.emarefa.net/detail/BIM-357940
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dawud, Iyad Rashid. Simultaneous high performance liquid chromatographic (HPLC) determination of ACE inhibitors in pharmaceutical formulations. (Master's theses Theses and Dissertations Master). Al-Ahliyya Amman University. (2013).
https://search.emarefa.net/detail/BIM-357940
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dawud, Iyad Rashid. (2013). Simultaneous high performance liquid chromatographic (HPLC) determination of ACE inhibitors in pharmaceutical formulations. (Master's theses Theses and Dissertations Master). Al-Ahliyya Amman University, Jordan
https://search.emarefa.net/detail/BIM-357940
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-357940
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر