Cell wall glycosidase activities and protein content variations during fruit development and ripening in three texture contrasted tomato cultivars

الملخص EN

Excessive softening is the main factor limiting fruit shelf life and storage.

It is generally acceptable now that softening of fruit which occurs during the ripening is due to synergistic actions of several enzymes on cell wall polysaccharides.

As a subject for this study, we have assayed some glycosidase activities using three tomato species (Lycopersicon esculentum) contrasted for their texture phenotypes; the cherry tomato line Cervil (Solanum lycopersicum var.

cerasiforme), a common taste tomato line Levovil (S.

lycopersicum Mill.) and VilB a modern line, large, firmer and with good storage capability.

Four glycosidase activities namely a-galactosidase, b-galactosidase, b-mannosidase and b-glucosidase were extracted from tomato’s cell wall of the three species.

Cell wall protein from fruits pericarp was extracted and compared among the three cultivars at the following stages; 14 days post anthesis (14DPA) fruit; 21 days post anthesis (21DPA), turning (breaker), red and over ripe.

When glycolytic activities were also compared among these cultivars at the precited development stages, gross variations were noticed from stage to stage and also from species to species in accordance with the fruit firmness status.

Interestingly, VilB cultivar, the firmer among the other two, though