Molecular diagnosis of plant viruses
العناوين الأخرى
التشخيص الجزيئي للفيروسات النباتية
المؤلفون المشاركون
Qamari, Safa Ghassan
Makkouk, Khalid
المصدر
Arab Journal of Plant Protection
العدد
المجلد 24، العدد 2 (31 ديسمبر/كانون الأول 2006)4ص.
الناشر
تاريخ النشر
2006-12-31
دولة النشر
لبنان
عدد الصفحات
4
التخصصات الرئيسية
الموضوعات
الملخص AR
-Proper virus identification is always the key in developing appropriate practical solutions to manage plant virus diseases.
Recent advances in biotechnology and molecular biology have played a significant role in the development of rapid, specific and sensitive diagnostic tests.
The use of ELISA, by employing either polyclonal or monoclonal antibodies, was a significant step in adding sensitivity and precision to virus detection.
The development of the tissue-blot immunoassay (TBIA), as a variant of ELISA, greatly simplified testing, reduced the cost and permitted virus testing at locations where facilities are limited or even absent.
Immuno Chromatographic Assay (ICA), is another ELISA variant which added speed to virus identification, where results can be obtained within 10-15 minutes, as compared to 2-3 hours for TBIA.
However, ICA is more expensive than TBIA.
The development of nucleic acid-based tools was another new dimension of virus detection.
The most common among these techniques are cDNA hybridization and polymerase chain reaction (PCR).
In addition, PCR can be used as a confirmatory test for TBIA, where processed blots can be cut individually and tested by PCR.
This proved to work well with both DNA and RNA plant viruses.
Furthermore, unprocessed plant tissue blots on nitrocellulose membrane represent a good sample for PCR amplification.
PCR products can also be used for cloning and subsequent sequencing which is extremely useful for identification of new viruses or virus strains.
الملخص EN
-Proper virus identification is always the key in developing appropriate practical solutions to manage plant virus diseases.
Recent advances in biotechnology and molecular biology have played a significant role in the development of rapid, specific and sensitive diagnostic tests.
The use of ELISA, by employing either polyclonal or monoclonal antibodies, was a significant step in adding sensitivity and precision to virus detection.
The development of the tissue-blot immunoassay (TBIA), as a variant of ELISA, greatly simplified testing, reduced the cost and permitted virus testing at locations where facilities are limited or even absent.
Immuno Chromatographic Assay (ICA), is another ELISA variant which added speed to virus identification, where results can be obtained within 10-15 minutes, as compared to 2-3 hours for TBIA.
However, ICA is more expensive than TBIA.
The development of nucleic acid-based tools was another new dimension of virus detection.
The most common among these techniques are cDNA hybridization and polymerase chain reaction (PCR).
In addition, PCR can be used as a confirmatory test for TBIA, where processed blots can be cut individually and tested by PCR.
This proved to work well with both DNA and RNA plant viruses.
Furthermore, unprocessed plant tissue blots on nitrocellulose membrane represent a good sample for PCR amplification.
PCR products can also be used for cloning and subsequent sequencing which is extremely useful for identification of new viruses or virus strains.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Makkouk, Khalid& Qamari, Safa Ghassan. 2006. Molecular diagnosis of plant viruses. Arab Journal of Plant Protection،Vol. 24, no. 2.
https://search.emarefa.net/detail/BIM-359840
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Makkouk, Khalid& Qamari, Safa Ghassan. Molecular diagnosis of plant viruses. Arab Journal of Plant Protection Vol. 24, no. 2 (Dec. 2006).
https://search.emarefa.net/detail/BIM-359840
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Makkouk, Khalid& Qamari, Safa Ghassan. Molecular diagnosis of plant viruses. Arab Journal of Plant Protection. 2006. Vol. 24, no. 2.
https://search.emarefa.net/detail/BIM-359840
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 138
رقم السجل
BIM-359840
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر