Prevalence of coagulase (coa)‎ gene and mec A gene of S. aureus isolated from bovine clinical mastitis

الملخص EN

S.

aureus is recognized worldwide as one of the frequent cause of intramammary infections in dairy cows.

In order to determine the prevalence of S.aureus bovine clinical mastitis as well as detection of coagulase and mec A genes of S,aureus , a total of 78 quarter milk samples were collected from clinical cases of mastitis after the investigation of 100 lactating Friesian cows at Ismailia Governorate.

By bacteriological examination the percentage of S.aureus in bovine clinical mastitis was (28.2%).

Ten isolates were subjected to PCR for detection of coagulase gene (coa) gene, (100%) were positive.

PCR protocol used for amplification and detection of (mec A) gene of MRSA as a confirm diagnosis of their resistance to methicillin antibiotic .

All MRSA isolates were 100% positive .

Finally, the prevalence of MRSA mec A gene was very low in comparison with coagulase gene.