The effect of different hormonal supplementations on pregnancy support and outcome in mice

الملخص EN

The effectiveness of different hormonal supplementations and durations of injection on eariy embryonic implantation and pregnancy outcome in mice were investigated.

One hundred and ninety six mothered mice were divided into two major groups depending on hormonal stimulation of estrous cycle of females either stimulated or non-stimulated.

Further each of major group was subdivided into two minor groups depending on period of injection during gestation either for lsr week or 1" and 2nd weeks.

Each minor group was divided according to type of hormone supplement including human chorionic gonadotrophin (3 1U), progesterone (0.07 mg), estradiol (0.035 mg) or other combinations between progesterone and human chorionic gonadotrophin or estradiol.

The main parameters were studied are gestation period, litter size and endometrium thickness of uterine horn.

The results of the present study observed that the administration of hormones have insignificant differences in the period of gestation as compared to its control groups.

Also, litter size and endometrium thickness of uterine horn were significantly (P< 3.05) increased as a result of hormonal treatments when compared to its control groups.

However, progesterone supplementation for ls: and 2nd weeks of gestation Period was best and significantly (P<0.01) increased litter size and endometrium .hickness of uterine horn without noticeable side effects.

It was concluded that the synchronization of ovarian stimulation and hormonal supplementation by using either progesterone alone or in combination with human chorionic gonadotrophin nay be have good results to improve normal embryonic implantation rate and ;nhanc.e pregnancy outcome.

Further hormonal and histological studies are ecommer.ded to investigate the effects of different hormonal supplementations on ;ndocrine and reproductive organs of pregnant female.