Dose the presentation of patients effect the biochemical characteristics and oxidative stress in polycystic ovary syndrome?

الملخص EN

The present work aimed to investigate the relationships between biochemical characteristics and oxidative stress status in patients with polycystic ovary syndrome.

Methods : We measured Glucose, Triglyceride, Insulin, luteinizing hormone (LH), follicle-stimulating hormone (FSH), LH / FSH, Prolactin, Total testosterone(TT), Free testosterone(FT), Free androgen index(FAI), Esteradiol(E2), Progesterone, Malondialdehyde (MDA),Reduced glutathione (GSH), Glutathione-S-transferase levels in 143 PCOS patients and in 37 normal individuals.

Used spectrophotometrical methods were used to determine Glucose, Triglyceride, Malondialdehyde (MDA), reduced glutathione (GSH), Glutathione-S-transferase levels.

Hormonal assay was determined by using a commercially available ELISA kits.

Results : Results pointed out that 111(78 %) and 32 (22 %) of the patients presented with and without hirsutism respectively.

These are significant rises in TT (p < 0.01), FT (p = 0.002), and GST (p < 0.05) levels in the group of patients with hirsutism when compared with those without hirsutism.

However, significant decrease of SHBG (p < 0.05) value was obtained during a comparable evaluation.

The investigated women were exhibited to have irregular and regular cycle respectively.

Data analysis revealed significant elevations of TG (p < 0.001), FIN (p < 0.001), LH (p < 0.001), LH / FSH (p < 0.001), FIA (p < 0.01) levels and significant decreases of SHBG (p < 0.01), GSH (p < 0.01) and GST (p < 0.01) levels in the group of patients with irregular cycle when compared with those of regular menstruation pattern.

To perceive the relationship of the infertility pattern, i.e., primary or secondary infertility with changes of the biochemical parameters patients categorized into two groups.

Ninety (63 %) and 53 (37 %) out of the 143 patients were presented with primary and secondary infertility respectively.

Data analysis of the two groups pointed out significant increases of TG (p < 0.001), FIN (p < 0.001), LH (p < 0.05), LH / FSH (p < 0.05), TT (p = 0.03), FAI (p < 0.001), and E2 (p < 0.05) in patients with primary infertility in comparison with those of secondary infertility.

Significant lowering of SHBG (p < 0.01), and GST (p = 0.003) levels were also observed during a comparable evaluation.

Other parameters failed to exhibit significant variations Conclusion : Our results revealed that PCOS is associated with imbalanced oxidative / antioxidative status.

The impact of androgens on the development of hirsutism.

Oxidative stress is also a directing factor in these patients, since it was observed to be high in association with hyperinsulinaemia.

GST levels was higher in patients with hirsutism relative to those without this complication.

Hyperandrogenism is associated with a rise of oxidative stress, thus GST activity may increase as a compensatory mechanism to protect against raised oxidative stress.