Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah Province, Iran

المؤلفون المشاركون

Abbasi, S.
Chehri, K.
Salleh, B.
Yli Mattila, T.
Reddy, K. R. N.

المصدر

Saudi Journal of Biological Sciences

العدد

المجلد 18، العدد 4 (31 ديسمبر/كانون الأول 2011)، ص ص. 341-351، 11ص.

الناشر

الجمعية السعودية لعلوم الحياة

تاريخ النشر

2011-12-31

دولة النشر

السعودية

عدد الصفحات

11

التخصصات الرئيسية

النبات

الموضوعات

الملخص EN

Fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in Kermanshah province, the largest area of cucurbits plantation in Iran.

Therefore, the objectives in this study were to isolate and identify disease-causing Fusarium spp.

from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships.

A total of 100 Fusarium isolates were obtained from diseased cucurbit plants collected from fields in different geographic regions in Kermanshah province, Iran.

According to morphological characters, all isolates were identified as Fusarium oxysporum, Fusarium proliferatum, Fusarium equiseti, Fusarium semitectum and Fusarium solani.

All isolates of the five Fusarium spp.

Were evaluated for their pathogenicity on healthy cucumber (Cucumis sativus) and honeydew melon (Cucumis melo) seedlings in the glasshouse.

F.

oxysporum caused damping-off in 20–35 days on both cucurbit seedlings tested.

Typical stem rot symptoms were observed within 15 days after inoculation with F.

solani on both seedlings.

Based on the internal transcribed spacer (ITS) regions of ribosomal DNA (rDNA) restriction fragment length polymorphism (RFLP) analysis, the five Fusarium species were divided into two major groups.

In particular, isolates belonging to the F.

solani species complex (FSSC) were separated into two RFLP types.

Grouping among Fusarium strains derived from restriction analysis was in agreement with criteria used in morphological classification.

Therefore, the PCR-ITS-RFLP method provides a simple and rapid procedure for the differentiation of Fusarium strains at species level.

This is the first report on identification and pathogenicity of major plant pathogenic Fusarium spp.

causing root and stem rot on cucurbits in Iran.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Chehri, K.& Salleh, B.& Yli Mattila, T.& Reddy, K. R. N.& Abbasi, S.. 2011. Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah Province, Iran. Saudi Journal of Biological Sciences،Vol. 18, no. 4, pp.341-351.
https://search.emarefa.net/detail/BIM-364553

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Chehri, K.…[et al.]. Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah Province, Iran. Saudi Journal of Biological Sciences Vol. 18, no. 4 (2011), pp.341-351.
https://search.emarefa.net/detail/BIM-364553

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Chehri, K.& Salleh, B.& Yli Mattila, T.& Reddy, K. R. N.& Abbasi, S.. Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah Province, Iran. Saudi Journal of Biological Sciences. 2011. Vol. 18, no. 4, pp.341-351.
https://search.emarefa.net/detail/BIM-364553

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 350-351

رقم السجل

BIM-364553