Formulation and evaluation of Guiera Senegalensis leaves extract as antimicrobial and antidiabetic capsule dosage form

مقدم أطروحة جامعية

al-Jumayli, Abd Allah Husayn Murshid

مشرف أطروحة جامعية

al-Tuhami, Mahjub Sharif
Abd al-Karim, Abd al-Karim Muhammad

الجامعة

جامعة أم درمان الإسلامية

الكلية

كلية الصيدلة

القسم الأكاديمي

قسم الصيدلانيات

دولة الجامعة

السودان

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2012

الملخص الإنجليزي

Guiera senegalensis plant (Gubiesh) is one of the common medicinal plants in Africa.

In Sudan leaves of Guiera senegalensis is used in folk medicine to treat many diseases including diabetes mellitus and certain infectious diseases.

Often used in the form of popular products prepared in ways such as water soaking or cooking (infusion or decoction), and these methods are associated with a lot of defects (safety dislike, and encourage of poor patient compliance) due to lack of application of good manufacturing practices.

To overcome of these defects, design or formulation of these traditional doses in other pharmaceutical dosage form such as capsules is recommended. The objective of this study was to formulate and evaluate the extract of G.

senegalensis leaves in capsule dosage form in a safe and effective dose to substitute that used in the traditional liquid dosage forms and which also must be meets conventional pharmaceutical standards. To realize this objective, the leaves of Guiera senegalensis were extracted by different methods of extraction (maceration, decoction, infusion and continuous extraction) using different solvents (water, chloroform, ethanol and methanol).

The ethanolic extract obtained by maceration (ethanol 96 %) was considered as the best extract for formulation as capsule dosage form due to the biological, economical (low cost than methanol and don’t need freeze drying as water extract) and stability reasons (the components of aqueous extract are unstable due to the fermentation process), while the other extracts were rejected for the opposite reasons.

The phytochemical screening test was carried for the ethanolic extract, and the results revealed the presence of flavonoids, tannins, saponins, Cumarins, triterpenes and alkaloids and absence of anthraquinones, cyanogenic and sterols.

Seven extracts of all obtained extracts were selected to evaluate their antimicrobial activity.

All extracts exhibited low to moderate activity against the tested organisms.

Then, the ethanolic extract (maceration in ethanol 96%) was selected to evaluate its hypoglycemic activity using isolated rat hemidiaphragm and the results showed that the ethanolic extract exhibited significant effect in glucose utilization especially in dose of 200 mg / ml. The ethanolic extract was mixed with suitable excipients and converted into granules using wet granulation method, the obtained granules were evaluate for their flowability.

The flowability of granules was excellent.

Three different formulations were prepared; formula 1 contained microcrystalline cellulose as diluent, formula 2 and formula 3 contained lactose as diluent, also formula 3 contained polyethylene glycol and tween 20 as surfactant and wetting agent respectively, and all formulations contained Croscarmellose as disintegrant, magnesium stearate and talc as lubricant and glidant respectively and then they were filled in capsules of size (0) using manual filling machine (plate method). The manufactured capsules were evaluated by quality control tests (weight variation, disintegration, and dissolution).

Formula 2 and 3 met the specifications of British pharmacopoeia, 2007, while the capsules of formula 1 failed in dissolution test. Finally, the capsules of formulae 2 and 3 were subjected to accelerated stability study and the results showed that the prepared capsules were stable and appeared maintenance in their physicochemical properties during the period of study and under the conditions of storage.

In general, the results showed that the ethanolic extract was suitable for formulation in capsule dosage form, and the formula 3 which contained polyethylene glycol 4000 and tween 20 was considered the best formula, due to its higher dissolution profile.

التخصصات الرئيسية

علم الصيدلة

الموضوعات

• الأدوية

عدد الصفحات

123

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-364597