Characteristics studies of 1251-anti total PSA antibody’s binding with prostate specific antigen (PSA)‎ in human uterus tumors

الملخص EN

-Two groups of uterus tumors (benign and malignant) postmenopausal patients were used to investigate the presence of prostate specific antigen (PSA) .

Preliminary experiments were performed to follow the binding of i2r>I-anti total PSA antibody with PSA in uterus tissues homogenales of the two groups with their corresponding antigen and found to be(8.8,7.1%) for benign and malignant tumors ,respectively .

An jmmunoRadioMetricAssay (IRMA) procedure was developed for measuring PSA in benign ana malignant uterus tumors homogenates.

The optimum conditions of the binding of 125I-artli total PSA antibody with PSA were as follows: PSA concentration (150,200 f.tg protein), tracer antibody concentration (125,250 jig protein), pH (7.6,7.2), temp (15,25°C) and time (1.5 hrs) for postmenopausal benign and malignant uterus tumors tissue homogenates, respectively.

The use of different concentrations of Na-1 and Mg"1 ions were shown to cause an increase in the binding at concentration of (125,75 mM) of NaH ions and (75,225 mM) of Mg2'ions for benign and malignant uterus tumors homogenates ,respectively, while the use of different concentrations of urea and polyethylene glycol (PEG) caused a decrease in the binding with the increase in the concentration of each of urea and PEG in the both cases.