Detection of mycobacterium tuberculosis in sputum using conventional methods and PCR

الملخص EN

We evaluated the Polymerase Chain Reaction (PCR) based test for the detection of Mycobacterium tuberculosis complex, the common deadly infectious disease.

and conventional methods smear stained with acid fast staining and cultures on Lowenstain–Jensen medium in 50 sputum samples which were collected from patients with symptoms of tuberculosis in nineveh governorate.

The result indicated that, 19 (38 %) were smear positive by ZN stain direct or traditional method, and 20 (40 %) were smear positive by ZN stain concentrated method, 17 (34 %) were smear positive by Kinyouns method, 21 (42 %) were LJ culture positive for M.

tuberculosis, and 22 (44 %) were given specific bands of amplified DNA in a garose (2 %) with lengthed specific fragment is 390bpand internal control 750 bp using MTB complex 390 / 750 IC from sacace biotechnologies kit.

This test is a valuable diagnostic tool for today s mycobacteriology laboratory.