Determination of glucose level as a parameter for cell inhibition by 5-flurouracil in human colonic cancer sw-480 cell line

الملخص EN

Colonic cancer (SW-480) cell line was cultivated on two microtiter-plates of 48 wells, by using the routine culture methods and all what needed from culture media and reagents.

One microtiter-plate of SW-480 cell line was exposed to serial double dilutions of 5FU starting from 80 μg / mL of 5FU for 12 hours incubation.

Optical density was measured after staining with crystal violet and growth inhibition percentage was calculated.

Second SW-480 cultured plate was also exposed to same 5-FU dilutions and incubation period, and then the glucose level was determined in the culture medium.

There was significant increase (P < 0.05) in the inhibition percent and glucose level in SW-480 culture media for 5FU concentrations starting from 2.5μg / ml and up word.

In conclusion : According to the result, determination of glucose concentration may be a valuable predictor for the antitumor drug response.