التخلص من بعض الفيروسات البطاطا باستخدام تقنيات العلاج الحراري و زراعة أطراف البراعم

الملخص EN

Two separate experiments were conducted to study the possibility of producing virus-free tissue cultured plants from sprouts of infected potato plantlcts and sprouted tubers (cv.

Desiree), by using heat therapy.

In the first experiment, four groups of plantlcts infected with Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX).

and Potato virus S (PVS) were exposed to alternating temperatures of 40°C and 25°C for a duration of 4 hours each and for a period of fifteen days.

The second experiment involved etiolated shoots incubated at constant temperature of 37 °C for the same period prior to isolation of shoot tips (3-4 mm) for the purpose of producing virus-free plants.

Exposing infected potato plantlcts to fluctuating temperatures resulted in 100% elimination of PLRV, PVY, PVX and 91.7% of PVS, as determined by ELISA tests.

Incubation at a temperature of 37°C resulted in 85, 82.5, 82.5 and 77.5% reduction of infection with the four viruses, respectively.

Potato plantlcts were able to tolerate high temperatures under the conditions of the experiment with a survival rate of 91.3%.

Thus, the use of fluctuating temperature regimes were very effective, compared to constant temperature, in controlling economically important viruses affecting the potato crop, especially PVS.