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The determination of those polypeptides of Newcastle disease virus involved in protection
المؤلف
المصدر
Mu'tah Journal for Research and Studies : Natural and Applied Sciences Series
العدد
المجلد 16، العدد 1 (31 مارس/آذار 2001)، ص ص. 107-118، 12ص.
الناشر
تاريخ النشر
2001-03-31
دولة النشر
الأردن
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
The objective of this study was to identify those polypeptides of Newcastle disease virus (NDV) that are involved in protection.
Six groups of 3-week old chickens were injected with either the Hemagglutinin-neuraminidase protein(HN), Fusion protein(F), Nucleoprotein(NP), Matrix protein(M), UV-inactivated NDV(UV-NDV) (positive control) or saline (negative control).
Blood samples were collected weekly for evaluating the humoral and cellular immune responses using Western blot, ELISA and a MTT lymphoblastogensis microassay.
Protective immunity was determined by clinical disease and virus shedding following challenge with NDV strain Texas GB.
Antibodies to UV-NDV, HN, F and NP were detected at 3 weeks post-vaccination by Western blot and ELISA.
Lymphocytes from all vaccinated birds, with the exception of the group vaccinated with the M polypeptide, demonstrated specific increased blastogenesis when stimulated with UV-NDV.
The maximum stimulation occurred with the group vaccinated with the HN polypeptide.
All vaccinated groups other than the UV-NDV, were susceptible to NDV infection and clinical disease when challenged.
It is hypothesized that conformational changes in polypeptides during isolation and purification may have altered the protective epitopes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Maraqah, Anwar Diyab. 2001. The determination of those polypeptides of Newcastle disease virus involved in protection. Mu'tah Journal for Research and Studies : Natural and Applied Sciences Series،Vol. 16, no. 1, pp.107-118.
https://search.emarefa.net/detail/BIM-377475
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Maraqah, Anwar Diyab. The determination of those polypeptides of Newcastle disease virus involved in protection. Mu'tah Journal for Research and Studies : Natural and Applied Sciences Series Vol. 16, no. 1 (2001), pp.107-118.
https://search.emarefa.net/detail/BIM-377475
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Maraqah, Anwar Diyab. The determination of those polypeptides of Newcastle disease virus involved in protection. Mu'tah Journal for Research and Studies : Natural and Applied Sciences Series. 2001. Vol. 16, no. 1, pp.107-118.
https://search.emarefa.net/detail/BIM-377475
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes appendices : p. 117-118
رقم السجل
BIM-377475
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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