Chemically induced mutagenesis in Aspergillus nidulans using cis-[Pd (biq)‎ Cl2] as compared with NTG and spontaneous mutations

الملخص EN

Dicliloro(212'-biquinolinc)palladium(I])J ctj-1 ]>dCbiq)Clj]» appears to be one of ilic most powerful mutagenic compounds yet discovered.

Mutations in at least eleven genes can result in potassium chlorate and/or bromate resistance in Aspergillus nidulans.

Various proportions or mutants from all types of generated chlorate resistant ones were obtained, The variation in proportion of different mutant cl asses was highly dependent on whether or not a chemically induced mutagenesis was employed and also on which nitrogen source is serving as sole N-sourcc with the toxic substancc for selection.

The three basic amino acids (histidine, lysine, and arginine) were the only servants, that were capable of generating various mutant types when selection was performed at 25° C, and were capable to gcncrale mutants carrying double resistance to chlorate and bromate whether or not the mutagen was used.

The greatest proportion of generated mutants was for utaD mutants.

The next highest proportion of mutants was for nirA group while nltA gene the closer neighbor to niaD genc is less prone to mutations than niaD.

The treatment, the N-source and the selection temperature significantly affect the generation of crn mutants.

The proportion of cnx mutants is far from that of niaD mutants and is positively affected by the basic amino acids.

Concerning cnx heterokaryons complementation groups, ctvcF group was much commoner among the rest and this was significantly dependent 011 the servants basic amino acids used for selection whether or not a mutagen was used.