Toxicological pathologic study of effect of various insulin doses in mice

الملخص EN

Thiis sttudy concenttrratte on tthe effffectt off syntthettiic iinsulliin iin llaborrattorry aniimalls,, 80 whiitte miice,, rrandomlly diiviided iintto ffourr maiin grroups off malles and ffemalles conttrroll – unttrreatted,, ttrreatted as llow dose,, iintterrmediiatted dose and hiigh dose,, tthen everry grroup allso diiviided iin ttwo subgrroups,, tthe ffiirrstt subgrroup was giiven Acttrrapiid and second subgrroup was giiven Monottrrade ,, tthe ttestted matterriiall was giiven daiilly subcuttaneouslly unttrreatted conttrroll grroup ..

The iinjjecttiion was conttiinued unttiill aniimalls werre kiilllled afftterr 24 weeks off ttrreattmentt ..Clliiniicall,, macrroscopiicall and miicrroscopiicall sttudiies off experriimenttall miice iin comparriison wiitth tthe conttrroll grroup and tthe rresulltt werre as ffollllows :: 1-- IInjjectted miice showed norrmall acttiiviitty,, wiitth iincrrease body weiightt and llow ffood consumpttiion iin comparriison tto conttrrolls..

On severrall occasiions,, ttherre werre attttacks off hypogllycaemiia wiitth coma iin ttrreatted miice..

2-- Macrroscopiic examiinattiion off ttrreatted miice showed prresence off severrall llayerrs off adiipose ttiissue arround viiscerrall orrgans ,, iin perrii rrenall rregiion ,, iin tthe serrosall arreas off smallll iinttesttiine ,, iin perrii brronchiiall rregiion,, iin subcuttaneouslly ,, perrii pancrreattiic rregiion ,, splleniic enllarrgementt iin scatttterred ttrreatted aniimalls ,, 3-- Hiisttopatthollogiicall changes werre seen as ffatttty iinffiillttrrattiion // degenerrattiion iin tthe lliiverr,, diillatted corrttiicall ttubulles iin tthe kiidney and enllarrged zona ffasciicullatte off tthe adrrenalls..

IIn addiittiion tto attrrophy and // orr depllettiion off pancrreattiic iisllett off llangerrhans,, allso exttrramedullllarry haemopoiiesiis and // orr attrrophy off llymphoiid ttiissue iin tthe splleen,, wiitth perrii ffolllliicullarr deposiitts off amylloiid iin Splleniic whiitte pullp and iin gllomerrullii off kiidneys off grroup B..

Furrttherrmorre,, ttherre werre eviidence off stteattiittiis ((ffocall iinffllammattiion off adiipose ttiissue)) and enllarrgementt off zona ffasiicullatta iin adrrenalls..