Effects of achillea santolina extracts and fractions on human platelet aggregation in vitro and on rat arteriovenous shunt thrombosis in vivo

الملخص EN

Background : Achillea santolina (As), a plant grown in Irak, has been used in herbal medicine for thousands of years.

It has anti-inflammatory properties, but its antiplatelet or antithrombotic activities have not been explored.

Methods : The effects of As leaf crude extracts and fractions extracted with chloroform (F1), diethyl ether (F2), ethyl acetate (F3) and water (F4) were assessed in vitro on platelet human aggregation induced by ADP and collagen and on a rat arteriovenous shunt thrombosis model in vivo after 10 days oral administration (10 mg / kg / d).

Results : A.

santolina crude extract dose-dependently inhibited in vitro ADP and collagen-induced human platelet aggregation (maximal inhibition respectively 34.4 ± 2.9 % and 78.3 ± 2.5 %).

This effect was mostly contained in the diethylester (F2) fraction ; F1 and F3 had about half the effect, and F4 was devoid of antiaggregant effect.

Achillea Santolina extracts given orally daily for 10 days at the dose of 10mg / kg / day decreased thrombus weight but not significantly.

The discrepancy between clear dose-dependent in vitro effects on ADP and Collageninduced human platelet aggregation and unclear in vivo effects in rats raises questions as to active concentrations, intestinal resorption and / or metabolisation of active compounds.