Detection of genomic instability in oral squamous cell carcinoma using random amplified polymorphic DNA based on polymerase chain reaction method (RAPD-PCR)‎

الملخص EN

Background : Oral squamous cell carcinoma is an invasive epithelial neoplasm, occurred most commonly in alcoholic and tobacco using adults.

The present study is aimed to identify the genomic instability in OSCC patients using random amplified polymorphic DNA (RAPD), a polymerase chain reaction (PCR) based technique.

Materials and methods : Twenty five blocks of formalin fixed paraffin embedded tissue was used as malignant DNA source and five sample of healthy DNA obtained from the oral tissue and blood.

Using DNA extraction kit (Geneaid minikit) and eleven random sequencing primers to visualize the amplifications pattern under UV.

Results : The primer detectability of genomic instability ranged from 21 % in well differentiated OSCC to 68 % in poorly differentiated OSCC.

CasesT8 and T13 showed highest genomic instability (75 %).

The results determined numbers of genomic instabilities among OSCC patients by comparing the pattern of amplifications of the primers in both malignant and healthy DNA.

Conclusions : High significance correlation between primers detection rate and histopathological grade of OSCC.

Further larger studies are needed to : 1) Obtain RAPD markers useful for OSCC for early diagnosis ; 2) investigate different genes directly involved in the etiology of OSCC ; 3) analyze chromosomal instability among OSCC patients.