Molecular identification of entamoeba histolytica parasite by using actin and amebapore-A genes

الملخص EN

Objective : The present study was conducted in the province of Al- Najaf, for the period from April 2013 until November 2013, which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore–A genes in stool samples by using the real-time PCR technique.

Methodology : The study included, collect ( 104 ) stool samples from patients with diarrhea arrivals to Al-Sadar Medical City Hospital, Al- Zahra Maternity and Children Hospital, Al-Manathera hospital and Al-Sajjad Hospital.

Results : The results of the real-time PCR revealed, the presence of E.

histolytica parasites in (25) stool samples out of the (104) samples, the rate (24 %).

Conclusion : In light of the results obtained through this study we concludes that the efficiency of real-time -PCR technique large and rapid in diagnosis of E.

histolytica parasites in stool samples.

Recommendation : The study of gene expression of Actin and Amebapore–A genes, specific for E.

histolytica parasite.