![](/images/graphics-bg.png)
A quantitative Real Time PCR based method for the detection of Phytophthora infestans causing Late blight of potato, in infested soil
المؤلفون المشاركون
Husayn, Touseef
Singh, Bir Pal
Anwar, Fayruz
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 21، العدد 4 (30 سبتمبر/أيلول 2014)، ص ص. 380-386، 7ص.
الناشر
تاريخ النشر
2014-09-30
دولة النشر
السعودية
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
A fast and simple polymerase chain reaction method has been developed for detection of Phytophthora infestans oospores, the causal agent of Late blight of Potato in soil.
The method involves the disruption of oospores by grinding dry soil, using abrasive properties, in the presence of glass powder and skimmed milk powder within a short time.
The latter prevents loss of DNA by adsorption to soil particles or by degradation and reduces the co-extraction of PCR inhibitors with the DNA.
After phenol/chloroform extraction; the DNA is suitable for direct PCR amplification without a precipitation step.
This amplification leads to detection of pathogen in infested soils before planting of crop.
The real-time PCR assay we describe is highly sensitive and specific, and has several advantages over conventional PCR assays used for P.
infestans detection to confirm positive inoculum level in potato seeds and elsewhere.
With increasing amounts of standard DNA templates, the respective threshold cycle (Ct) values were determined and a linear relationship was established between these Ct values and the logarithm of initial template amounts.
The method is rapid, cost efficient, and when combined with suitable internal controls can be applied to the detection and quantification of P.
infestans oospores on a large-scale basis
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Husayn, Touseef& Singh, Bir Pal& Anwar, Fayruz. 2014. A quantitative Real Time PCR based method for the detection of Phytophthora infestans causing Late blight of potato, in infested soil. Saudi Journal of Biological Sciences،Vol. 21, no. 4, pp.380-386.
https://search.emarefa.net/detail/BIM-393794
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Husayn, Touseef…[et al.]. A quantitative Real Time PCR based method for the detection of Phytophthora infestans causing Late blight of potato, in infested soil. Saudi Journal of Biological Sciences Vol. 21, no. 4 (Sep. 2014), pp.380-386.
https://search.emarefa.net/detail/BIM-393794
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Husayn, Touseef& Singh, Bir Pal& Anwar, Fayruz. A quantitative Real Time PCR based method for the detection of Phytophthora infestans causing Late blight of potato, in infested soil. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 4, pp.380-386.
https://search.emarefa.net/detail/BIM-393794
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 385-386
رقم السجل
BIM-393794
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)